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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > E4F1 Antibody (monoclonal) (M03)

E4F1 Antibody (monoclonal) (M03)

Catalog # Availability Size / Price Inquiry
AMM03476G 100 μg / $495

E4F1 Antibody (monoclonal) (M03)

Brand

Leading Biology

Catalog Number

AMM03476G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality E4F1 Antibody (monoclonal) (M03).

Molecular Weight

83496 Da

Host

mouse

Species Reactivity

Human

Target

E4F1 (NP_004415.2, 685 a.a. ~ 784 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone

4B10

Isotype

IgG2a Kappa

GeneID

UniProt ID

Summary

The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IF, E

Dilution

WB~~1:500~1000

Images

Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .

Western Blot analysis of E4F1 expression in transfected 293T cell line by E4F1 monoclonal antibody (M03), clone 4B10.Lane 1: E4F1 transfected lysate(83.5 KDa).Lane 2: Non-transfected lysate.

Immunofluorescence of monoclonal antibody to E4F1 on HeLa cell . [antibody concentration 10 ug/ml]

Specification

Quantity

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