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E4F1 Antibody (monoclonal) (M03)Brand |
Leading Biology | Catalog Number |
AMM03476G |
Product Type |
Monoclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality E4F1 Antibody (monoclonal) (M03).
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Molecular Weight |
83496 Da
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Host |
mouse
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Species Reactivity |
Human
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Target |
E4F1 (NP_004415.2, 685 a.a. ~ 784 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
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Clone |
4B10
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Isotype |
IgG2a Kappa
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GeneID |
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UniProt ID |
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Summary |
The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence.
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IF, E
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Dilution |
WB~~1:500~1000
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Images |
Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) . Western Blot analysis of E4F1 expression in transfected 293T cell line by E4F1 monoclonal antibody (M03), clone 4B10.Lane 1: E4F1 transfected lysate(83.5 KDa).Lane 2: Non-transfected lysate. Immunofluorescence of monoclonal antibody to E4F1 on HeLa cell . [antibody concentration 10 ug/ml] |
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Specification |
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Quantity |
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