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Polyclonal Antibodies >
ETO polyclonal antibodyBrand |
Leading Biology | Catalog Number |
APR00372G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ETO polyclonal antibody.
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Molecular Weight |
67566 Da
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Cellular Localization |
Antigen Cellular Localization:
Nucleus {ECO:0000255|PROSITE- ProRule:PRU00440, ECO:0000269|PubMed:10973986}. Note=Colocalizes with ATN1 in discrete nuclear dots
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Host |
Rabbit
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Target |
ETO
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Isotype |
Rabbit IgG
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Symbol |
AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2
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GeneID |
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UniProt ID |
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Function |
Transcription regulator that excerts its function by binding to histone deacetylases and transcription factors. Can repress transactivation mediated by TCF12.
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Summary |
ETO is a transcriptional regulator which belongs to the myeloid translocation gene family. ETO exerts its function by interaction with transcription factors bound to promoters and binding to histone deacetylases. It recruits a range of corepressors to facilitate transcriptional repression. The t (8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. This translocation produces a chimeric gene made up of the 5’-region of AML1 and the 3’-region of the ETO gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.
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Form |
Liquid |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
E
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Images |
ChIP assays were performed using SKNO-1 cells, the antibody and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used. An ELISA was performed using a serial dilution of the antibody. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1300. |
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Specification |
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Quantity |
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