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HLA-B Antibody (N-term)

APR06103G Product Datasheet Protocol

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APR06103G		100 μl / $495

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Leading Biology Inc.

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HLA-B Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR06103G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality HLA-B Antibody (N-term).
Molecular Weight	40428 Da
Cellular Localization	Antigen Cellular Localization: Membrane; Single-pass type I membrane protein
Host	Rabbit
Species Reactivity	Human
Immunogen	62-90 aa
Target	This HLA-B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human HLA-B.
Isotype	Rabbit Ig
Symbol	HLAB
UniProt ID	P03989
Function	Involved in the presentation of foreign antigens to the immune system.
Summary	HLA-B is involved in the presentation of foreign antigens to the immune system.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, FC, E
Dilution	FC~~1:10~50 WB~~1:1000 IHC-P~~1:10~50
Images	Flow cytometric analysis of human peripheral blood lymphocytes cells using HLA-B Antibody (N-term) (green, APR06103G) compared to an isotype control of rabbit IgG (blue). APR06103G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody. Flow cytometric analysis of human peripheral blood lymphocytes cells using HLA-B Antibody (N-term) (green, APR06103G) compared to an isotype control of rabbit IgG (blue). APR06103G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody. Western blot analysis of lysates from CEM, HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody (N-term)(APR06103G). APR06103G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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