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LAG3 Antibody (Center)

APR05829G Product Datasheet Protocol

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APR05829G		100 μl / $495

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Leading Biology Inc.

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LAG3 Antibody (Center)

Brand	Leading Biology	Catalog Number	APR05829G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality LAG3 Antibody (Center).
Molecular Weight	57449 Da
Cellular Localization	Antigen Cellular Localization: Membrane; Single-pass type I membrane protein
Host	Rabbit
Species Reactivity	Human, Mouse
Immunogen	103-132 aa
Target	This LAG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human LAG3.
Isotype	Rabbit Ig
Symbol	FDC
GeneID	3902
UniProt ID	P18627
Function	Involved in lymphocyte activation. Binds to HLA class-II antigens.
Summary	Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IHC-P, E
Dilution	IHC-P~~1:10~50 FC~~1:25 WB~~1:1000
Images	AP6987C staining LAG3 in human thymus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Overlay histogram showing Jurkat cells stained with AP6987C (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6987C, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-LAG3 Antibody (Center) at 1:1000 dilution Lane 1: human ovary lysate Lane 2: mouse liver lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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