• Account
  • Account
    Inquiries
    0

    Inquired items

    0 items total Subtotal:$ 0
  • 会员中心
    Cart

    Added items

    0items total Subtotal:$ 0

All Departments

E2F-1 Antibody

Catalog # Availability Size / Price Inquiry
AMM03208G 100 μl / $495

E2F-1 Antibody

Brand

Leading Biology

Catalog Number

AMM03208G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality E2F-1 Antibody.

Molecular Weight

70 KDa

Cellular Localization

Antigen Cellular Localization: Nucleus.

Host

Mouse

Species Reactivity

Human

Symbol

RBBP3

GeneID

UniProt ID

Function

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.

Summary

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, ICC, IP

Dilution

WB~~1:500 ICC~~1:100 IP~~1:500

Images

Western blot detection of E2F-1 in C6,Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.

Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-E2F-1 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).

Immunoprecipitation analysis of Hela cell lysates using E2F-1 mouse mAb.

Specification

Quantity

Related Products

Select Brand Catalog No. Product Name Pack Size Type Field of Research Specification Quantity Price(USD)
1 Leading Biology AMM07977G SPRY1 / Sprouty 1 Antibody (clone 3H4) 50 μg Monoclonal Antibodies
$545.00 Add Ask
2 Leading Biology AMM00137G CD79a (B-Cell Marker) Antibody - With BSA and Azide 50 ug Monoclonal Antibodies
$395.00 Add Ask
3 Leading Biology APG02467G CCK4 / PTK7 Antibody (clone 4F9) 50 μl Monoclonal Antibodies
$495.00 Add Ask
4 Leading Biology AMM05365G DC-SIGN Antibody [5D7] 0.1 mg Monoclonal Antibodies
$695.00 Add Ask
5 Leading Biology AMM01402G Vimentin (Mesenchymal Cell Marker) Antibody - With BSA and Azide 50 ug Monoclonal Antibodies
$395.00 Add Ask
6 Leading Biology APR08280G LTA4H / LTA4 Antibody (clone 9G8) 50 μl Monoclonal Antibodies
$495.00 Add Ask
Contact Us

This item has successfully been added to your cart!

You have 0 item in your cart

Your inquiry has successfully been added to your list!

You have 0 item in your inquiry list