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Phospho-CCNB1(S35) Antibody

Catalog # Availability Size / Price Inquiry
APR05338G 100 μl / $495

Phospho-CCNB1(S35) Antibody

Brand

Leading Biology

Catalog Number

APR05338G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Phospho-CCNB1(S35) Antibody.

Molecular Weight

48337 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Host

Rabbit

Species Reactivity

Human

Target

This CCNB1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S35 of human CCNB1.

Isotype

Rabbit Ig

Symbol

CCNB

GeneID

UniProt ID

Function

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Summary

The protein encoded by this gene is a regulatory proteininvolved in mitosis. The gene product complexes with p34(cdc2) toform the maturation-promoting factor (MPF). Two alternativetranscripts have been found, a constitutively expressed transcriptand a cell cycle-regulated transcript, that is expressedpredominantly during G2/M phase. The different transcripts resultfrom the use of alternate transcription initiation sites. [providedby RefSeq].

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

DB, WB, E

Dilution

WB~~1:1100 DB~~1:500

Images

Western blot analysis of lysate from Hela cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody (Cat. APR05338G) or CCNB1-S9 Antibody.Lysate at 15ug per lane.APR05338G was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.

Dot blot analysis of CCNB1 Antibody (Phospho S35) Phospho-specific Pab (Cat. APR05338G) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Specification

Quantity

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