> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > YWHAG Antibody (N-term)Brand |
Leading Biology | Catalog Number |
APR05095G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality YWHAG Antibody (N-term).
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Molecular Weight |
28303 Da
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Cellular Localization |
Antigen Cellular Localization:
Cytoplasm.
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Host |
Rabbit
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Species Reactivity |
Human, Mouse
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Immunogen |
63-92 aa
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Target |
This YWHAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human YWHAG.
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Isotype |
Rabbit Ig
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GeneID |
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UniProt ID |
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Function |
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
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Summary |
YWHAG belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, FC, E
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Dilution |
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
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Images |
YWHAG Antibody (N-term) (Cat. APR05095G) western blot analysis in A431,Hela cell line,mouse brain and rat brain lysates (35ug/lane).This demonstrates the YWHAG antibody detected the YWHAG protein (arrow).
Formalin-fixed and paraffin-embedded human brain tissue reacted with YWHAG Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
YWHAG Antibody (N-term) (Cat. APR05095G) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Specification |
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Quantity |
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Tel: 1-661-524(LBI)-0262
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