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GNG12 Antibody (C-Term)

APR04951G Product Datasheet Protocol

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APR04951G		100 μl / $495

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Leading Biology Inc.

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GNG12 Antibody (C-Term)

Brand	Leading Biology	Catalog Number	APR04951G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GNG12 Antibody (C-Term).
Molecular Weight	8006 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side
Host	Rabbit
Species Reactivity	Human, Mouse
Target	This GNG12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 37-68 amino acids from human GNG12.
Isotype	Rabbit Ig
GeneID	55970
UniProt ID	Q9UBI6
Function	Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.
Summary	Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IHC-P, WB, E
Dilution	IHC-P~~1:25 WB~~1:2000
Images	APR04951G staining GNG12 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-GNG12 Antibody (C-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: DU145 whole cell lysate Lane 3: mouse brain lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 8 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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