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USP47 Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR04849G 100 μl / $495

USP47 Antibody (Center)

Brand

Leading Biology

Catalog Number

APR04849G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality USP47 Antibody (Center).

Molecular Weight

157311 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm.

Host

Rabbit

Species Reactivity

Human

Target

This USP47 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 588-620 amino acids from the Central region of human USP47.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). Acts as a regulator of cell growth and genome integrity. May also indirectly regulates CDC25A expression at a transcriptional level.

Summary

Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). Acts as a regulator of cell growth and genome integrity. May also indirectly regulates CDC25A expression at a transcriptional level.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

IHC-P, WB, E

Dilution

IHC-P~~1:25 WB~~1:2000

Images

APR04849G staining USP47 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes : Anti-USP47 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: human testis lysates Lane 3: U-87 MG whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 157 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Specification

Quantity

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