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PPP1R13L Antibody (N-Term)

Catalog # Availability Size / Price Inquiry
APR04846G 100 μl / $495

PPP1R13L Antibody (N-Term)

Brand

Leading Biology

Catalog Number

APR04846G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PPP1R13L Antibody (N-Term).

Molecular Weight

89091 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Nucleus. Note=Predominantly cytoplasmic but also nuclear

Host

Rabbit

Species Reactivity

Human

Target

This PPP1R13L antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 134-166 amino acids from human PPP1R13L.

Isotype

Rabbit Ig

Symbol

IASPP, NKIP1, PPP1R13BL, RAI

GeneID

UniProt ID

Function

Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis.

Summary

Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

IF, WB, E

Dilution

IF~~1:25 WB~~1:2000

Images

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma cell line) cells labeling Pdx1 with APR04846G at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

All lanes : Anti-PPP1R13L Antibody (N-Term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: NCI-H1299 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 89 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Specification

Quantity

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