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RAB20 Antibody

AMM02559G Product Datasheet Protocol

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AMM02559G		100 μl / $495

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Leading Biology Inc.

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RAB20 Antibody

Brand	Leading Biology	Catalog Number	AMM02559G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RAB20 Antibody.
Molecular Weight	26277 Da
Cellular Localization	Antigen Cellular Localization: Golgi apparatus. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane; Lipid-anchor; Cytoplasmic side Note=Highly enriched on apical endocytic structures in polarized epithelial cells of kidney proximal tubules (By similarity) Recruited to phagosomes containing S.aureus or M.tuberculosis
Host	Mouse
Species Reactivity	Human
Target	This RAB20 antibody is generated from a mouse immunized with recombinant protein from human RAB20.
Clone	1694CT210.218.54
Isotype	IgG2a
GeneID	55647
UniProt ID	Q9NX57
Function	Plays a role in apical endocytosis/recycling. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes.
Summary	Plays a role in apical endocytosis/recycling. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	FC, WB, E
Dilution	FC~~1:25 WB~~1:2000-1:4000
Images	Overlay histogram showing Hela cells stained with AMM02559G(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM02559G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-RAB20 Antibody at 1:2000-1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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