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HBA2 Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR06167G 100 μl / $495

HBA2 Antibody (Center)

Brand

Leading Biology

Catalog Number

APR06167G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality HBA2 Antibody (Center).

Molecular Weight

15258 Da

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

100-128 aa

Target

This HBA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-128 amino acids from the Central region of human HBA2.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Involved in oxygen transport from the lung to the various peripheral tissues.

Summary

HBA2 located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

FC~~1:25 WB~~1:1000 IHC-P~~1:50~100

Images

Overlay histogram showing K562 cells stained with APR06167G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR06167G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Anti-HBA2 Antibody (Center) at 1:2000 dilution + human fetal heart lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of HBA2 Antibody (Center) (Cat. APR06167G) in mouse liver tissue lysates (35ug/lane). HBA2 (arrow) was detected using the purified Pab.

Specification

Quantity

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