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Polyclonal Antibodies >
PSMB9 Antibody (C-term)Brand |
Leading Biology | Catalog Number |
APR06094G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PSMB9 Antibody (C-term).
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Molecular Weight |
23264 Da
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Cellular Localization |
Antigen Cellular Localization:
Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00809}. Nucleus
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
193-219 aa
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Target |
This PSMB9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-219 amino acids from the C-terminal region of human PSMB9.
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Isotype |
Rabbit Ig
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Symbol |
LMP2, PSMB6i, RING12
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GeneID |
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UniProt ID |
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Function |
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
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Summary |
PSMB9 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, FC, E
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Dilution |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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Images |
All lanes : Anti-PSMB9 Antibody (C-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of PSMB9 Antibody (C-term) (Cat. APR06094G) in HL-60 cell line lysates (35ug/lane). PSMB9 (arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PSMB9 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Specification |
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Quantity |
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