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SMYD3 Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR05893G 100 μl / $495

SMYD3 Antibody (Center)

Brand

Leading Biology

Catalog Number

APR05893G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SMYD3 Antibody (Center).

Molecular Weight

49097 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Nucleus. Note=Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M

Host

Rabbit

Species Reactivity

Human

Immunogen

209-236 aa

Target

This SMYD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-236 amino acids from the Central region of human SMYD3.

Isotype

Rabbit Ig

Symbol

ZMYND1, ZNFN3A1

GeneID

UniProt ID

Function

Histone methyltransferase. Specifically methylates 'Lys- 4' and 'Lys-5' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.

Summary

SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, FC, E

Dilution

WB~~1:1000 FC~~1:10~50

Images

Western blot analysis of lysate from HT-29 cell line, using SMYD3 Antibody (Center)(APR05893G). APR05893G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot analysis of SMYD3 antibody (Center) (Cat.APR05893G) in CEM cell line lysates (35ug/lane). SMYD3 (arrow) was detected using the purified Pab.

SMYD3 Antibody (Center) (Cat. APR05893G) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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