> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > Cyclin A (CCNA2) Antibody (N-term)Brand |
Leading Biology | Catalog Number |
APR05876G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Cyclin A (CCNA2) Antibody (N-term).
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Molecular Weight |
48551 Da
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Cellular Localization |
Antigen Cellular Localization:
Nucleus. Cytoplasm. Note=Cytoplasmic when associated with SCAPER
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
51-84 aa
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Target |
This Cyclin A (CCNA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-84 amino acids from the N-terminal region of human Cyclin A (CCNA2).
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Isotype |
Rabbit Ig
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Symbol |
CCN1, CCNA
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GeneID |
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UniProt ID |
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Function |
Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
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Summary |
CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, FC, E
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Dilution |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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Images |
Western blot analysis of anti-CCNA2(N-term) Pab (Cat.APR05876G) in K562 cell line lysates (35ug/lane). CCNA2(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with Cyclin A (CCNA2) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of HepG2 cells using Cyclin A (CCNA2) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Specification |
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Quantity |
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| 6 | Leading Biology | APR03502G | IGHG1 Antibody (Center) | 100 μl | Polyclonal Antibodies |
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Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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