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SPP1 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR05768G 100 μl / $495

SPP1 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR05768G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SPP1 Antibody (C-term).

Molecular Weight

35423 Da

Cellular Localization

Antigen Cellular Localization: Secreted.

Host

Rabbit

Species Reactivity

Human

Immunogen

273-301 aa

Target

This SPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-301 amino acids from the C-terminal region of human SPP1.

Isotype

Rabbit Ig

Symbol

BNSP, OPN

GeneID

UniProt ID

Function

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Summary

SPP1 binds tightly to hydroxyapatite. The protein appears to form an integral part of the mineralized matrix and probably important to cell-matrix interaction. It acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Images

Western blot analysis of SPP1 Antibody (C-term) (Cat. APR05768G) in NCI-H460 cell line lysates (35ug/lane). SPP1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma with SPP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of NCI-H292 cells using SPP1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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