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TRAP Antibody (N-term)

APR05766G Product Datasheet Protocol

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APR05766G		100 μl / $495

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Leading Biology Inc.

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TRAP Antibody (N-term)

Brand	Leading Biology	Catalog Number	APR05766G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality TRAP Antibody (N-term).
Molecular Weight	29274 Da
Cellular Localization	Antigen Cellular Localization: Cell membrane; Single-pass type II membrane protein
Host	Rabbit
Species Reactivity	Human
Immunogen	33-62 aa
Target	This TRAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human TRAP.
Isotype	Rabbit Ig
Symbol	CD40L, TNFSF5, TRAP
GeneID	959
UniProt ID	P29965
Function	Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Summary	TRAP is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in its gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IF, IHC-P, FC, E
Dilution	WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50 FC~~1:10~50
Images	All lanes : Anti-TRAP Antibody (N-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: THP-1 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of TRAP antibody (N-term) (Cat. APR05766G) in NCI-H460 cell line lysates (35ug/lane). TRAP (arrow) was detected using the purified Pab. TRAP Antibody (N-term) (Cat. APR05766G) IHC analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRAP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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