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PXN Antibody (Y118)

Catalog # Availability Size / Price Inquiry
APR05754G 100 μl / $495

PXN Antibody (Y118)

Brand

Leading Biology

Catalog Number

APR05754G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PXN Antibody (Y118).

Molecular Weight

64505 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex. Note=Colocalizes with integrins at the cell periphery.

Host

Rabbit

Species Reactivity

Human

Immunogen

94-125 aa

Target

This PXN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-125 amino acids from human PXN.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

Summary

PXN is a cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. PXN contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:10~50

Images

All lanes : Anti-PXN Antibody (Y118) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of PXN(arrow) using rabbit polyclonal PXN Antibody (Y118) (Cat.APR05754G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with PXN gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PXN Antibody (Y118) (Cat.APR05754G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specification

Quantity

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