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BTG1 Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR05512G 100 μl / $495

BTG1 Antibody (Center)

Brand

Leading Biology

Catalog Number

APR05512G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality BTG1 Antibody (Center).

Molecular Weight

19209 Da

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

92-118 aa

Target

This BTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-118 amino acids from the Central region of human BTG1.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Anti-proliferative protein.

Summary

BTG1 is a member of an anti-proliferative gene family that regulates cell growth and differentiation. Expression of this gene is highest in the G0/G1 phases of the cell cycle and downregulated when cells progressed through G1. The encoded protein interacts with several nuclear receptors, and functions as a coactivator of cell differentiation. This locus has been shown to be involved in a t(8;12)(q24;q22) chromosomal translocation in a case of B-cell chronic lymphocytic leukemia.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:50~100

Images

All lanes : Anti-BTG1 Antibody (Center) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

(LEFT)Western blot analysis of BTG1 Antibody (Center) (Cat. APR05512G) in MCF-7 cell line lysates (35ug/lane).BTG1 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of BTG1 Antibody (Center) (Cat. APR05512G) in mouse NIH-3T3 tissue lysates (35ug/lane).BTG1 (arrow) was detected using the purified Pab.

BTG1 Antibody (Center) (Cat. APR05512G) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BTG1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Specification

Quantity

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