> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > Roundabout 4 Antibody (N-term)Brand |
Leading Biology | Catalog Number |
APR05496G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Roundabout 4 Antibody (N-term).
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Molecular Weight |
107457 Da
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
236-264 aa
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Target |
This Roundabout 4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the N-terminal region of human Roundabout 4.
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Isotype |
Rabbit Ig
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GeneID |
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UniProt ID |
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Function |
Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins (By similarity).
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Summary |
Roundabout 4 is receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. This protein may mediate the inhibition of primary endothelial cell migration by Slit proteins.
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC-P, FC, E
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Dilution |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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Images |
Roundabout 4 Antibody (N-term) (Cat. APR05496G) western blot analysis in 293,K562,HL-60 cell line lysates (35ug/lane).This demonstrates the Roundabout4 antibody detected the Roundabout4 protein (arrow).
Roundabout 4 Antibody (N-term) (Cat. APR05496G) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Roundabout 4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Roundabout 4 Antibody (N-term) (Cat. APR05496G) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Specification |
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Quantity |
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