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Phospho-cJun(S63) Antibody

Catalog # Availability Size / Price Inquiry
APR05116G 100 μl / $495

Phospho-cJun(S63) Antibody

Brand

Leading Biology

Catalog Number

APR05116G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Phospho-cJun(S63) Antibody.

Molecular Weight

35676 Da

Cellular Localization

Antigen Cellular Localization: Nucleus.

Host

Rabbit

Species Reactivity

Human

Target

This cJun Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S63 of human cJun.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.

Summary

This gene for the cJun protein is the putative transforming gene of avian sarcoma virus 17. The protein is highly similar to the viral protein, and interacts directly with specific target DNA sequences to regulate gene expression. The gene for this protein is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

DB, WB, IHC-P, E

Dilution

DB~~1:500 WB~~1:1000 IHC-P~~1:50~100

Images

Dot blot analysis of anti-Phospho-cJun-S63 Antibody (Cat.APR05116G) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Western blot analysis of anti-Phospho-cJun-pS63 Pab (Cat. APR05116G) in mouse kidney tissue lysate (35ug/lane). cJun-pS63 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Specification

Quantity

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