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CRISP1 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR04235G 100 μl / $495

CRISP1 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR04235G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CRISP1 Antibody (C-term).

Molecular Weight

28481 Da

Cellular Localization

Antigen Cellular Localization: Note=Located in the lumen and epithelium of distal ductus efferentes and epididymal ducts, and on the postacrosomal region of the sperm head

Host

Rabbit

Species Reactivity

Human

Immunogen

162-191 aa

Target

This CRISP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-191 amino acids from the C-terminal region of human CRISP1.

Isotype

Rabbit Ig

Symbol

AEGL1

GeneID

UniProt ID

Function

May have a role in sperm-egg fusion and maturation.

Summary

Fertilization consists of a sequence of specific cell-cellinteractions culminating in the fusion of the sperm and egg plasmamembranes. Recognition, binding, and fusion occur through theinteraction of complementary molecules that are localized tospecific domains of the sperm and egg plasma membranes. In thesperm, the postacrosomal region or equatorial segment is involvedin sperm-egg plasma membrane fusion. The protein encoded by thisgene is a member of the cysteine-rich secretory protein (CRISP)family. This protein is expressed in the epididymis, is secretedinto the epididymal lumen, and binds to the postacrosomal region ofthe sperm head where it plays a role at fertilization in sperm-eggfusion through complementary sites localized on the egg surface.Two isoforms are encoded by transcript variants of this gene.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, E

Dilution

WB~~1:1000

Images

Western blot analysis of lysate from MDA-MB-231 cell line, using CRISP1 Antibody (C-term)(Cat. APR04235G). APR04235G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

Specification

Quantity

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