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CNOT4 Antibody

Catalog # Availability Size / Price Inquiry
APR03986G 100 μl / $495

CNOT4 Antibody

Brand

Leading Biology

Catalog Number

APR03986G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CNOT4 Antibody.

Molecular Weight

63510 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Nucleus

Host

Rabbit

Species Reactivity

Human

Target

This CNOT4 antibody is generated from rabbits immunized with human CNOT4 recombinant protein.

Isotype

Rabbit Ig

Symbol

NOT4

GeneID

UniProt ID

Function

Has E3 ubiquitin ligase activity. Involved in activation of the JAK/STAT pathway.

Summary

CNOT4 has intrinsic E3 ubiquitin ligase activity. Binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective.The CCR4-NOT complex functions as a global regulator of RNA polymerase II transcription.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

Images

Western blot analysis of CNOT4 Antibody (Cat. APR03986G) in MCF-7, K562, 293 cell line lysates (35ug/lane). CNOT4 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with CNOT4 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

CNOT4 Antibody (Cat. APR03986G) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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