> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > SPOPL Antibody (Center)Brand |
Leading Biology | Catalog Number |
APR03736G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality SPOPL Antibody (Center).
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Molecular Weight |
44647 Da
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Cellular Localization |
Antigen Cellular Localization:
Nucleus.
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Host |
Rabbit
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Species Reactivity |
Human
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Immunogen |
221-249 aa
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Target |
This SPOPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-249 amino acids from the Central region of human SPOPL.
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Isotype |
Rabbit Ig
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GeneID |
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UniProt ID |
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Function |
Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, but with relatively low efficiency. Cullin-RING-based BCR (BTB-CUL3- RBX1) E3 ubiquitin-protein ligase complexes containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL are less efficient than ubiquitin ligase complexes containing only SPOP. May function to down-regulate the activity of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes that contain SPOP.
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Summary |
In complex with a cullin, may act in ubiquitination and proteasomal degradation processes (By similarity).
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
FC, WB, IHC-P, E
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Dilution |
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
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Images |
SPOPL Antibody (Center) (Cat. APR03736G) western blot analysis in uterus tumor cell line lysates (35ug/lane).This demonstrates the SPOPL antibody detected the SPOPL protein (arrow).
SPOPL Antibody (Center) (Cat. APR03736G)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPOPL Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
SPOPL Antibody (Center) (Cat. APR03736G) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis. |
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Specification |
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Quantity |
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| 6 | Leading Biology | APR03502G | IGHG1 Antibody (Center) | 100 μl | Polyclonal Antibodies |
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Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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