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ID1 Antibody (Center)

Catalog # Availability Size / Price Inquiry
APR03608G 100 μl / $495

ID1 Antibody (Center)

Brand

Leading Biology

Catalog Number

APR03608G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ID1 Antibody (Center).

Molecular Weight

16133 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm. Nucleus.

Host

Rabbit

Species Reactivity

Human, Rat

Immunogen

66-93 aa

Target

This ID1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-93 amino acids from the Central region of human ID1.

Isotype

Rabbit Ig

Symbol

BHLHB24, ID

GeneID

UniProt ID

Function

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).

Summary

The protein encoded by this gene is a helix-loop-helix(HLH) protein that can form heterodimers with members of the basicHLH family of transcription factors. The encoded protein has no DNAbinding activity and therefore can inhibit the DNA binding andtranscriptional activation ability of basic HLH proteins with whichit interacts. This protein may play a role in cell growth,senescence, and differentiation. Two transcript variants encodingdifferent isoforms have been found for this gene. [provided byRefSeq].

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, IF, FC, E

Dilution

WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50 FC~~1:10~50

Images

Anti-ID1 Antibody (Center) at 1:2000 dilution + PC-12 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 16 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-ID1 Antibody (Center) at 1:2000 dilution + PC-12 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 16 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from HepG2, MCF-7, U-2OS cell line (from left to right), using ID1 Antibody (Center)(APR03608G). APR03608G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Specification

Quantity

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