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TCF21 Antibody (C-term)

APR03582G Product Datasheet Protocol

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APR03582G		100 μl / $495

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Leading Biology Inc.

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TCF21 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR03582G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality TCF21 Antibody (C-term).
Molecular Weight	19715 Da
Cellular Localization	Antigen Cellular Localization: Nucleus.
Host	Rabbit
Species Reactivity	Human
Immunogen	144-173 aa
Target	This TCF21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-173 amino acids from the C-terminal region of human TCF21.
Isotype	Rabbit Ig
Symbol	BHLHA23, POD1
GeneID	6943
UniProt ID	O43680
Function	Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis. May play a role in the specification or differentiation of one or more subsets of epicardial cell types.
Summary	TCF21 encodes a transcription factor of the basichelix-loop-helix family. The TCF21 product is mesoderm specific,and expressed in embryonic epicardium, mesenchyme-derived tissuesof lung, gut, gonad, and both mesenchymal and glomerular epithelialcells in the kidney. Two transcript variants encoding the sameprotein have been found for this gene.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, FC, E
Dilution	WB~~1:1000 FC~~1:10~50
Images	Western blot analysis of lysates from Hela cell line mouse spleen tissue lysate(from left to right), using TCF21 Antibody (C-term)(APR03582G). APR03582G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. TCF21 Antibody (C-term) (Cat. APR03582G) western blot analysis in 293 cell line and human placenta tissue lysates (35ug/lane).This demonstrates the TCF21 antibody detected the TCF21 protein (arrow). TCF21 Antibody (C-term) (Cat. APR03582G) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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