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General 25OHVD/25-OH Vitamin D ELISA KitBrand |
Leading Biology | Catalog Number |
EK00740E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00740E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (25-OH Vitamin D) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 25-OH Vitamin D The ELISA analytical biochemical technique of the EK00740Ee kit is based on 25-OH Vitamin D antibody-25-OH Vitamin D antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 25-OH Vitamin D antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, 25-OH Vitamin D. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Assay Type |
Competitive
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Detection Method |
Spectrophotometrical
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Reactivity |
General
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
1.27nmol/L
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Assay Range |
3.12-200nmol/L
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
25OHVD
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Function |
Low blood calcidiol (25-hydroxy-vitamin D) can result from avoiding the sun. Deficiency results in impaired bone mineralization, and leads to bone softening diseases.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
Calcifediol,Calcidiol
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Images |
Standard curve of the product EK00740E. |
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Specification |
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Quantity |
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