>
Antigen, Antibodies, ELISA, Western Blot >
Immunoassay Kits >
ELISA Kit >
General PGI2/Prostacyclin ELISA KitBrand |
Leading Biology | Catalog Number |
EK00728E |
Product Type |
ELISA Kit | Field of Research |
|
Product Overview |
EK00728E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Prostacyclin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Prostacyclin The ELISA analytical biochemical technique of the EK00728Ee kit is based on Prostacyclin antibody-Prostacyclin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Prostacyclin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Prostacyclin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
|
||
Cellular Localization |
Prostacyclin is produced in endothelial cells from prostaglandin H2 (PGH2) by the action of the enzyme prostacyclin synthase. Although prostacyclin is considered an independent mediator, it is called PGI2 (prostaglandin I2) in eicosanoid nomenclature, and is a member of the prostanoids (together with the prostaglandins and thromboxane).
|
||
Assay Type |
Competitive
|
||
Detection Method |
Spectrophotometrical
|
||
Reactivity |
General
|
||
Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
|
||
Sensitivity |
10 pg/mL
|
||
Assay Range |
31.2-2000 pg/mL
|
||
Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
|
||
Other Reagents Required |
Deionized or distilled water
|
||
Gene Name |
PGI2
|
||
Function |
Prostacyclin (PGI2) chiefly prevents formation of the platelet plug involved in primary hemostasis (a part of blood clot formation). It does this by inhibiting platelet activation. It is also an effective vasodilator. Prostacyclin's interactions in contrast to thromboxane (TXA2), another eicosanoid, strongly suggest a mechanism of cardiovascular homeostasis between the two hormones in relation to vascular damage.
|
||
Form |
ELISA Kits |
||
Storage & Stability |
Store at +4°C. Please refer to protocols.
|
||
Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
|
||
Images |
Standard curve of the product EK00728E. |
||
Specification |
|||
Quantity |
|
Select | Brand | Catalog No. | Product Name | Pack Size | Type | Field of Research | Specification | Quantity | Price(USD) | |
1 | Leading Biology | EK00487E | Human DCN/Decorin ELISA Kit | 96T | ELISA Kit |
|
$645.00 | Add Ask | ||
2 | Leading Biology | EK00726E | General HS/Heparan sulfate ELISA Kit | 96T | ELISA Kit |
|
$745.00 | Add Ask | ||
3 | Leading Biology | EK00038E | General E2/Estradiol ELISA Kit | 96T | ELISA Kit |
|
$545.00 | Add Ask | ||
4 | Leading Biology | EK0003L | Exendin-4 (Exenatide) ADA | 96T | ELISA Kit |
|
$895.00 | Add Ask | ||
5 | Leading Biology | EK00003E | General Testosterone/Testosterone ELISA Kit | 96T | ELISA Kit |
|
$545.00 | Add Ask | ||
6 | Leading Biology | EK00793E | General GSH/Glutathione ELISA Kit | 96T | ELISA Kit |
|
$745.00 | Add Ask |
Complete this form and click send to ask us a question, request a quote or simply say hello.
You have 0 item in your cart
You have 0 item in your inquiry list