> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Mouse Pparg/Peroxisome proliferator-activated receptor gamma ELISA KitBrand |
Leading Biology | Catalog Number |
EK00668E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00668E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Peroxisome proliferator-activated receptor gamma) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Peroxisome proliferator-activated receptor gamma The ELISA analytical biochemical technique of the EK00668E kit is based on Peroxisome proliferator-activated receptor gamma antibody-Peroxisome proliferator-activated receptor gamma antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Peroxisome proliferator-activated receptor gamma antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Peroxisome proliferator-activated receptor gamma. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Nucleus Cytoplasm Redistributed from the nucleus to the cytosol through a MAP2K1/MEK1-dependent manner (By similarity). NOCT enhances its nuclear translocation.
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Mouse
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.083 ng/mL
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Assay Range |
0.156-10 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
Pparg
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UniProt ID |
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Function |
Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (PubMed:19041764).
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
PPAR-gamma,Nuclear receptor subfamily 1 group C member 3,Nr1c3
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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