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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human FADS2/Fatty acid desaturase 2 ELISA Kit

Human FADS2/Fatty acid desaturase 2 ELISA Kit

Catalog # Availability Size / Price Inquiry
EK00595E 96T / $695

Human FADS2/Fatty acid desaturase 2 ELISA Kit

Brand

Leading Biology

Catalog Number

EK00595E

Product Type

ELISA Kit

Field of Research

Product Overview

EK00595E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Fatty acid desaturase 2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Fatty acid desaturase 2 The ELISA analytical biochemical technique of the EK00595E kit is based on Fatty acid desaturase 2 antibody-Fatty acid desaturase 2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Fatty acid desaturase 2 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Fatty acid desaturase 2. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).

Cellular Localization

Endoplasmic reticulum membrane Multi-pass membrane protein

Assay Type

Sandwich

Detection Method

Spectrophotometrical

Reactivity

Human

Sample Type

This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Sensitivity

0.1 ng/mL

Assay Range

0.312-20 ng/mL

Kit Components

Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.

Other Reagents Required

Deionized or distilled water

Gene Name

FADS2

MIM

UniProt ID

Function

Acts as a fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 6 of the fatty acyl chain. Involved in biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors. Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleate (GLA) (18:3n-6) and stearidonate (18:4n-3), respectively (PubMed:12713571). Subsequently, in the biosynthetic pathway of HUFA n-3 series, desaturates tetracosapentaenoate (24:5n-3) to tetracosahexaenoate (24:6n-3), which is then converted to docosahexaenoate (DHA)(22:6n-3), an important lipid for nervous system function (By similarity). Desaturates palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum (PubMed:12713571). Also desaturates (11E)-octadecenoate (trans-vaccenoate)(18:1n-9), a metabolite in the biohydrogenation pathway of LA (18:2n-6) (By similarity).

Form

ELISA Kits

Storage & Stability

Store at +4°C. Please refer to protocols.

Applications

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Synonyms

Delta(6) fatty acid desaturase,D6D,Fatty acid desaturase 2

Images

Standard curve of the product EK00595E.

Specification

Quantity

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