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Human LPL/Lipoprotein lipase ELISA KitBrand |
Leading Biology | Catalog Number |
EK00588E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00588E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Lipoprotein lipase) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Lipoprotein lipase The ELISA analytical biochemical technique of the EK00588E kit is based on Lipoprotein lipase antibody-Lipoprotein lipase antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Lipoprotein lipase antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Lipoprotein lipase. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Cell membrane Lipid-anchor GPI-anchor Secreted Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles (By similarity).
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
7.8 pg/mL
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Assay Range |
31.2-2000 pg/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
LPL
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MIM |
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UniProt ID |
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Function |
The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL) (PubMed:27578112). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
LPL,LIPD
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Images |
Standard curve of the product EK00588E. |
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Specification |
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Quantity |
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