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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human FLT4/Vascular endothelial growth factor receptor 3 ELISA Kit

Human FLT4/Vascular endothelial growth factor receptor 3 ELISA Kit

Catalog # Availability Size / Price Inquiry
EK00544E 96T / $645

Human FLT4/Vascular endothelial growth factor receptor 3 ELISA Kit

Brand

Leading Biology

Catalog Number

EK00544E

Product Type

ELISA Kit

Field of Research

Signal Transduction

Product Overview

EK00544E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Vascular endothelial growth factor receptor 3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Vascular endothelial growth factor receptor 3 The ELISA analytical biochemical technique of the EK00544E kit is based on Vascular endothelial growth factor receptor 3 antibody-Vascular endothelial growth factor receptor 3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Vascular endothelial growth factor receptor 3 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Vascular endothelial growth factor receptor 3. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).

Cellular Localization

Isoform 3 Secreted Cytoplasm

Assay Type

Sandwich

Detection Method

Spectrophotometrical

Reactivity

Human

Sample Type

This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Sensitivity

0.16 ng/mL

Assay Range

0.312-20 ng/mL

Kit Components

Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.

Other Reagents Required

Deionized or distilled water

Gene Name

FLT4

MIM

UniProt ID

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.

Form

ELISA Kits

Storage & Stability

Store at +4°C. Please refer to protocols.

Applications

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Synonyms

VEGFR-3,Fms-like tyrosine kinase 4,FLT-4,Tyrosine-protein kinase receptor FLT4,VEGFR3

Images

Standard curve of the product EK00544E.

Specification

Quantity

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