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Human ANGPTL4/Angiopoietin-related protein 4 ELISA KitBrand |
Leading Biology | Catalog Number |
EK00243E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00243E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Angiopoietin-related protein 4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Angiopoietin-related protein 4 The ELISA analytical biochemical technique of the EK00243E kit is based on Angiopoietin-related protein 4 antibody-Angiopoietin-related protein 4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Angiopoietin-related protein 4 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Angiopoietin-related protein 4. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Secreted Secreted Extracellular space Extracellular matrix The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4 (By similarity).
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.044 ng/mL
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Assay Range |
0.156-10 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
ANGPTL4
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MIM |
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UniProt ID |
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Function |
Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
Angiopoietin-like protein 4,Hepatic fibrinogen/angiopoietin-related protein,HFARP,ARP4,HFARP,PGAR,PP1158,PSEC0166,UNQ171/PRO197
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Images |
Standard curve of the product EK00243E. |
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Specification |
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Quantity |
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