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Human A2M/Alpha-2-macroglobulin ELISA KitBrand |
Leading Biology | Catalog Number |
EK00226E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00226E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Alpha-2-macroglobulin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Alpha-2-macroglobulin The ELISA analytical biochemical technique of the EK00226E kit is based on Alpha-2-macroglobulin antibody-Alpha-2-macroglobulin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Alpha-2-macroglobulin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Alpha-2-macroglobulin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Secreted
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
3.9 ng/mL
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Assay Range |
7.8-500 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
A2M
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MIM |
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UniProt ID |
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Function |
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
Alpha-2-M,C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5,CPAMD5,FWP007
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Images |
Standard curve of the product EK00226E. |
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Specification |
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Quantity |
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