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Human ACE/ Angiotensin-converting enzyme ELISA KitBrand |
Leading Biology | Catalog Number |
EK00111E |
Product Type |
ELISA Kit | Field of Research |
Neurosciences |
Product Overview |
EK00111Ee is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Prostaglandin E2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Prostaglandin E2 The ELISA analytical biochemical technique of the EK00111Ee kit is based on Prostaglandin E2 antibody-Prostaglandin E2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Prostaglandin E2 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Prostaglandin E3. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Cell membrane Single-pass type I membrane protein Cytoplasm Detected in both cell membrane and cytoplasm in neurons.
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.067 ng/mL
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Assay Range |
0.156-10 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
ACE
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MIM |
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UniProt ID |
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Function |
Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
ACE,Dipeptidyl carboxypeptidase I,Kininase II,CD143,DCP,DCP1
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Images |
Standard curve of the product EK00111E. |
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Specification |
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Quantity |
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