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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > General T3/Triiodothyronine ELISA Kit

General T3/Triiodothyronine ELISA Kit

Catalog # Availability Size / Price Inquiry
EK00002E 96T / $545

General T3/Triiodothyronine ELISA Kit

Brand

Leading Biology

Catalog Number

EK00002E

Product Type

ELISA Kit

Field of Research

Product Overview

EK00002E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Triiodothyronine) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Triiodothyronine The ELISA analytical biochemical technique of the EK00002Ee kit is based on Triiodothyronine antibody-Triiodothyronine antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Triiodothyronine antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Triiodothyronine. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).

Cellular Localization

The T3 (and T4) bind to nuclear receptors, thyroid receptors. T3 (and T4) are very lipophilic and able to pass through the phospholipid bilayers of target cells. The lipophilicity of T3 (and T4) requires their binding to the protein carrier thyroid-binding protein (TBG) [thyroxine-binding globulins, thyroxine binding prealbumins, and albumins] for transport in the blood. The thyroid receptors bind to response elements in gene promoters, thus enabling them to activate or inhibit transcription. The sensitivity of a tissue to T3 is modulated through the thyroid receptors.

Assay Type

Competitive

Detection Method

Spectrophotometrical

Reactivity

General

Sample Type

This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Sensitivity

35 pg/mL

Assay Range

78-5000 pg/mL

Kit Components

Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.

Other Reagents Required

Deionized or distilled water

Gene Name

T3

Function

Triiodothyronine, C15H12I3NO4, also known as T3, is a thyroid hormone. It affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate.

Form

ELISA Kits

Storage & Stability

Store at +4°C. Please refer to protocols.

Applications

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Images

Standard curve of the product EK00002E.

Specification

Quantity

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