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  • RNA interfering
    RNA interference (RNAi gene interference, siRNA experiments). RNAi is the post-transcriptional  gene silencing phenomenon, which is caused by double-stranded RNA (DsRNA) homologous to the target gene sequence. Small interfering RNA (siRNA) degrades mRNA of the same sequence and blocks the expression of the corresponding gene. Abiocenter provide SiRNA fragments chemically synthesizing and RNA vector (ShRNA) construction services. Only name or gene ID of target genes are needs for the clients. We will help to screen the intervention sites. At least one pair of effective SiRNA and more than ...
  • How to select the primary antibody
    1. Analyze the experimental method of the experimental application Generally, antibody specifications will list the types of assays that the antibody has been tested for, such as: WB, IHC, ICC, ELISA, FCM analysis, etc. If the type of application is not mentioned in the specification, it does not imply that the antibody is not suitable for this type of experiment, it is merely indicating that this anybody has not been verified by the analytical test. In order to avoid the risk of experimentation, when the application method is not mentioned in the manual, you can apply for the test kit ...
  • Definition and classification of the antibodies
    1. antibody definition Antibody (immunoglobulin is not just an antibody) is a large Y-shaped protein secreted by plasma cells (effector B cells) and used by the immune system to identify and neutralize foreign substances such as bacteria and viruses. It is found in body fluids such as blood of vertebrates, and the cell membrane surface of its B cells. Antibodies can recognize a unique feature of a particular foreign object called as antigen. Related concepts Antigen (Ag): Any substance that induces an immune response. The foreign molecule can be identified by immuno...
  • How to select secondary antibody
    Secondary antibody: The secondary antibody is an antibody prepared in another host that binds to the primary antibody or the fragment of the primary a...
  • Western Blot Proteins Sample Preparation
    Western Blot Proteins Sample Preparation Before doing western blot, it’s crucial to extract the proteins. It almost contributes to both success...
  • ELISpot FAQs
    1. Which kind of ELISpot plate do you recommend? Answer: We recommend the ELISpot plate with PVDF membrane and pretreated with ethanol. It coul...
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