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  • Protein Expression and Purification Services
    Introduction Leading Biology strives to provide advanced platforms for protein expression and purification service. As a leading biotechnology service supplier, we offer a variety of protein expression and purification systems for high level expression and sample purification. The preparation of high quality, high purity and high-fidelity protein has become a key step in many experiments. Therefore, we deeply understand the importance of building a convenient and easy to use protein high expression and purification system. With our experienced expertise and unique well optimized protocols, we are able to evaluate efficiently and identify the optimal expression condition for a candidate protein to meet our customer’s special requirements. In order to make sure each protein sample the most efficient and closest to the natural host expression system, our company has set up various types of protein expression system. Here we’d like to invite you to review our growing range of expression systems resulting from our experience in cloning, overexpression and purification. Related Services Contact Information ...
  • Whole cell-based protein expression system
    Introduction The cell-based membrane protein expression system provided by our company, is based on the transfection of recombinant target protein expression plasmids. The diversity expression platform includes all kinds of protein expression sources, including bacteria, yeast, insect cells and mammalian cell lines, Customers only need to provide the information of related target proteins (gene sequence, amino acid sequence, source, protein solubility, etc.). We can start from the first step of downstream protein expression, and perform vector construction, expression, purification, stabilization, even the crystallization of the membrane protein. Under the efforts of our excellent R & D team, we have developed a series of new reagents that could inhibit the degradation, maintain the full-length, natural conformation and the maximum bioactivity of the protein, we also do optimization according to different target proteins. Our customized membrane proteins can meet the requirements of downstream antibody screening, drug discovery, structural determination, function and mechanism research. Comparison and summary Category ...
  • Ubiquitination
    Ubiquitination Ubiquitination (also known as ubiquitylation or ubiquitinylation) is an enzymatic post-translational modification in which a ubiquitin protein is attached to a substrate protein. This process most commonly binds the last amino acid of ubiquitin (glycine 76) to a lysine residue on the substrate.  The ubiquitin proteasome system The ubiquitin-proteasome system mediates 80%-85% protein degradation in eukaryotes. In addition to being involved in protein degradation, ubiquitination is also involved in almost all life activities, including cell cycle regulation, proliferation, apoptosis, differentiation, transcriptional regulation, gene expression, transcriptional regulation, signal transmission, injury repair, and inflammation immunity. Ubiquitination is associated with the pathogenesis of diseases such as cancer and cardiovascular diseases. Therefore, it has become a new target for new drug development. Our Advantages High-throughput: identify and quantify up to thousands of proteins at once H...
  • Acetylation
    Brief Introduction Of Protein Acetylation Acetylation refers to a reaction that introduces an acetyl functional group into a chemical compound, in which the hydrogen atom of a hydroxyl group is replaced by an acetyl group (CH3 CO) to yield a specific ester, the acetate. Protein acetylation commonly has two different forms. In humans, almost (80%-90%) proteins become co-translationally acetylated at their Nα-termini of the nascent polypeptide chains. Another type is typically acetylated on lysine residues. Protein acetylation plays a crucial role in regulating transcriptional activity. Acetylation complexes (such as CBP/p300) or deacetylation complexes (such as HDAC) can be recruited to DNA-bound transcription factors (TF) in response to signaling pathways. Histone hyper-acetylation by histone acetyl-transferases (HATs) is associated with transcriptional activation, presumably by remodeling nucleosomal structure into an open conformation more accessible to transcription complexes. Conversely, histone de-acetylation is associated with transcriptional repression reversing the chromatin remodeling process. Several transcriptional co-activators and co-r...
  • Methylation
    Methylation  Protein methylation is a post-translational modification (PTM) process, in which highly specific enzymes called methyltransferases are responsible for the addition of methyl groups to a targeted molecule and S-adenosyl methionine (SAM) as the primary donor of methyl group. Protein methylation commonly occurs on arginine, lysine, histidine, proline, and carboxyl groups. Protein methylation plays an important role in modulating cellular and biological processes, including transcriptional regulation, RNA processing, metabolism and signal transduction. The workflow of protein methylation analysis In addition, we also provide bioinformatics services in Protein Post-translational Modification Analysis. We have optimized our protocol to enable more fast and sensitive services for methylation analysis.  Our Advantages High-throughput: Identify and quantify up to thousands of proteins at once 100% coverage: Use 2-3 enzymes for protein digestion to ensure full-coverage Ability to analyze low abundance ubiquitin-proteins Constantly optimized and ...
  • Phosphorylation
    Phosphorylation Phosphorylation is an important post-translational modification (PTM) that affects protein’s shape, intracellular behavior and function. Kinase and phosphatase remove or add phosphate groups, mainly serine, threonine and tyrosine residues. These amino acids all contain a nucleophilic group that reacts with adenosine triphosphate (ATP), replacing the oxygen on the terminal phosphorus and ejecting adenosine diphosphate (ADP). Although kinases and phosphatases work in all living cells, they are particularly active in eukaryotes, where phosphorylation is one of about six post-translational modifications. Approximately 30% of proteins in eukaryotic cells undergo phosphorylation, which means that there are huge, untapped pathways for pharmacological intervention in almost all serious human diseases. For pharmaceutical companies that produce about 30 approved kinase inhibitor drugs, this idea has not been lost. Small molecule tyrosine kinase inhibitors include imatinib (used to treat leukemia) and gefinitib (used to treat leukemia), and gefitinib (used for breast cancer and lung cancer) can block signals that promote cancer...
  • Single Molecule Protein Detection
    Introduction Many systems are either dynamically or stationary inhomogeneous and the inhomogeneities, especially dynamic inhomogeneities, are the key to understand their interactions and functions.  Ensemble measurements naturally can only yield the mean value of the ensemble. Single-molecule trajectories on the other hand are direct records of the fluctuation that contain detailed dynamical and statistical information. Since trajectories may vary for different members of the ensemble the average trajectory contains less information, the ensemble might not even show fluctuations at all as they could be averaged out during the time span of the experiment. Single Molecule Protein Detection Single molecule array testing is a powerful new technique that is orders of magnitude more sensitive than standard sandwich based immunoassay techniques. Traditional ELISA measurements are limited to pg/ml levels of detection.  How It Works Simoa is based upon the isolation of individual immunocomplexes on paramagnetic beads using standard ELISA reagents. The main difference between Simoa and conventional immunoassays lies...
  • Protein Detection and Immunoassay
    Immunoassay development Immunoassays enable the sensitive and specific measurement of analytes in complex biologic matrices and thus play a central role in drug discovery and development. We are well versed in what it takes to develop and validate an immunoassay in the regulated bioanalytical setting through our deep understanding of the science, statistical analysis demands and experience in the complex regulatory landscape that exists today. These attributes, coupled with our comprehensive project management and the available capacity to support high-throughput platforms and complex methods in a compliant manner, position us well to service all your testing needs. How can LB help you on your immunoassay development? Read the information on our website.  Contact us to briefly discuss your project and determine how our capabilities can meet your needs. Request a confidentiality agreement if you need. Review, sign, and send it back to us, or send us your confidentiality agreement for us to review. Discuss the project with us in further detail so we can prepare a reliable quote. Accept the quote...
  • Protein Expression and Purification Service
    Introduction Leading Biology strives to provide advanced platforms for protein expression and purification service. As a leading biotechnology service supplier, we offer a variety of protein expression and purification systems for high level expression and sample purification. The preparation of high quality, high purity and high-fidelity protein has become a key step in many experiments. Therefore, we deeply understand the importance of building a convenient and easy to use protein high expression and purification system. With our experienced expertise and unique well optimized protocols, we are able to evaluate efficiently and identify the optimal expression condition for a candidate protein to meet our customer’s special requirements. In order to make sure each protein sample the most efficient and closest to the natural host expression system, our company has set up various types of protein expression system. Here we’d like to invite you to review our growing range of expression systems resulting from our experience in cloning, overexpression and purification. Related Services Contact Information ...
  • Bacillus subtilis Protein Expression
    Bacillus subtilis expression system introduction 1. Simple cell wall composition, the secreted protein products don’t contain endotoxin. 2. The ability to produce a large number of extracellular/target proteins, simple recovery and purification. 3. Having a high efficient secretory signal peptide and molecular chaperone system, could achieve high efficient secretory of target protein, avoid the formation of inclusion bodies. Advantages of the Bacillus subtilis expression service in Leading Biology 1. Having a variety of Bacillus subtilis expressing vectors, such as pHT101 and pHT254. 2. Having many different expressions hosts, such as intracellular expression host AS1, 1012, 168, secretory expression host WB800N, etc to fulfill different requirements of customers. 3. Could prepare high efficient competent Bacillus subtilis. Service process Classic case An influenza virus capsid protein, molecular weight 100kD, the customer provides genetic information, after genetic optimization, the vector was constructed and expressed in Bacillus subtilis 168 and 1012:     &n...
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