We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality NBCe1 (extracellular) antibody.

121343 Da

Rabbit

Mouse, Rat

Peptide (C)KE(S)VKFGGKLVGNN, corresponding to amino acid residues 672-685 of rat NBCe1 (Accession Q9JI66). 2nd extracellular loop, based on the 10 transmembrane topology.Not recommended for human samples. The antibody will recognize all known rat splice variants

SLC4 membrane transporter proteins play important roles in kidney acid–base regulation through their transport of bicarbonate (or carbonate), Na‏+, Cl-, and (possibly NH4+)1. These transporters differ in their substrate (Na+‏, Cl-) dependence, charge transport stoichiometry, cell-type and developmental expression, functional regulation, and protein–protein interactions. In mammals, SLC4 proteins are encoded by 10 different genes that share protein sequence homology and are grouped according to their functional properties.In humans, the electrogenic sodium bicarbonate cotransporter NBCe-1 is encoded by the SLC4A4 gene2. NBCe1-A has a large N-terminal cytoplasmic region, a lipid embedded transmembrane region, and a C-terminal cytoplasmic tail3. The N-terminal cytoplasmic region is tightly folded and is predicted to form a domain structure, unlike the freely aqueous accessible C-terminal cytoplasmic tail. NBCe1-A is composed of either 10 or 14 transmembrane domains. Based on the 10 transmembrane topology,a large extracellular loop (EL) is present between TM3 and TM4 and contains two glycosylated sites. The oligomeric state of the cotransporter is dimeric and each monomeric subunit has independent transport activity4.Mutations in NBCe1 cause autosomal recessive proximal renal tubular acidosis (pRTA). Patients with NBCe1 mutations have severe pRTA, growth and mental retardation, basal ganglia calcification, cataracts, corneal opacities (band keratopathy), glaucoma, elevated serum amylase and lipase, and defects in the enamel consistent with amelogenesis imperfect3. Mice with loss of NBCe1 have a more severe phenotype with marked volume deletion and decreased survival5.

Affinity purified antibody, Liquid

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

WB, LCI

WB~~1:200-1:2000 IHC~~1:400 ICC~~1:100

Electrogenic sodium bicarbonate cotransporter 1, Sodium bicarbonate cotransporter, NBC-like protein, Na(+)/HCO3(-) cotransporter, Solute carrier family 4 member 4, Slc4a4, Nbc, Nbc1, Nbce1, Rnbc1

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