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UBE2W Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR03844G 100 μl / $495

UBE2W Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR03844G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality UBE2W Antibody (C-term).

Molecular Weight

17331 Da

Cellular Localization

Antigen Cellular Localization: Nucleus. Note=In the nucleus, colocalizes with FANCL

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

105-133 aa

Target

This UBE2W antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-133 amino acids from the C-terminal region of human UBE2W.

Isotype

Rabbit Ig

Symbol

UBC16

GeneID

UniProt ID

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Transfers ubiquitin in complex with RING/U-box type E3s that do not have active site cysteine residues to form thioester bonds with ubiquitin, and preferentially ubiquitinates the N-terminus of substrates, such as ATXN3, STUB1 and SUMO2.

Summary

UBE2W accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes monoubiquitination and 'Lys-11'-linked polyubiquitination.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

WB~~1:1000 IHC-P~~1:10~50

Images

UBE2W Antibody (C-term) (Cat. APR03844G) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the UBE2W antibody detected the UBE2W protein (arrow).

UBE2W Antibody (C-term) (Cat. APR03844G) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the UBE2W antibody detected the UBE2W protein (arrow).

UBE2W Antibody (C-term) (Cat. APR03844G)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE2W Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Specification

Quantity

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