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ACTB Antibody

Catalog # Availability Size / Price Inquiry
APR03421G 100 μl / $495

ACTB Antibody

Brand

Leading Biology

Catalog Number

APR03421G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ACTB Antibody.

Molecular Weight

41737 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs

Host

Rabbit

Species Reactivity

Human

Target

This ACTB antibody is generated from rabbits immunized with a recombinant protein from human ACTB.

Isotype

Rabbit Ig

GeneID

UniProt ID

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Summary

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, FC, E

Dilution

WB~~1:500-1:1000 IHC-P~~1:50~100 FC~~1:10~50

Images

Western blot analysis of lysates from A431, HepG2 cell line (from left to right), using ACTB Antibody(Cat. APR03421G). APR03421G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

ACTB Antibody (Cat. APR03421G) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACTB Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

ACTB Antibody (Cat. APR03421G) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specification

Quantity

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