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IGHG1 Antibody (Center)

APR03502G Product Datasheet Protocol

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APR03502G		100 μl / $495

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Leading Biology Inc.

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IGHG1 Antibody (Center)

Brand	Leading Biology	Catalog Number	APR03502G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality IGHG1 Antibody (Center).
Molecular Weight	36106 Da
Cellular Localization	Antigen Cellular Localization: Secreted.
Host	Rabbit
Species Reactivity	Human
Immunogen	154-180 aa
Target	This IGHG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-180 amino acids from the Central region of human IGHG1.
Isotype	Rabbit Ig
UniProt ID	P01857
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, FC, E
Dilution	WB~~1:2000 FC~~1:25
Images	Anti-IGHG1 Antibody (Center) at 1:2000 dilution + human plasma lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-IGHG1 Antibody (Center) at 1:2000 dilution Lane 1: human fetal spleen lysate Lane 2: human fetal thymus lysate Lane 3: human spleen lysate Lane 4: human plasma lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Overlay histogram showing HL-60 cells stained with APR03502G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR03502G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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