We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Human H4 Histamine Receptor (extracellular) antibody.

44496 Da

Rabbit

Human

Peptide HTLFEWDFGKEIC, corresponding to amino acids 75-87 of human H4 Histamine Receptor (Accession Q9H3N8).

Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1 The biological effects of histamine are mediated through four receptors (H1- H4 Histamine receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.H4 Histamine Receptor couples to Gi/G0 proteins and receptor activation leads to inhibition of adenylate cyclase, mobilisation of calcium from intracellular stores and activation of the mitogen-activated protein kinase (MAPK) cascade. 1, 2 H4 Histamine Receptor is largely expressed in haemopoietic cells including mast cells, eosinophils, dendritic cells and T lymphocytes. H4 Histamine Receptors modulate eosinophil migration and the selective recruitment of mast cells leading to amplification of histamine-mediated immune responses. In addition, H4 Histamine Receptors are involved in dendritic cell activation and the regulation of T lymphocyte cytokine production.2, 3These studies indicate that H4 Histamine Receptor is an attractive therapeutic target for the treatment of inflammatory disorders, such as allergy, asthma, chronic pruritus and autoimmune diseases.2, 3 Abgent is pleased to offer a highly specific antibody directed against an epitope located at the extracellular 1st loop of the human H4 Histamine receptor. Anti-Human H4 Histamine Receptor (extracellular) antibody (#AG1224) can be used in western blot and indirect flow cytometry applications, and recognizes H4 Histamine receptor from human samples.

Affinity purified antibody, Liquid

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

FC, WB

FC~~1:35 WB~~1:200-1:2000 IF~~1:20

Histamine H4 receptor, H4R, HH4R, AXOR35, G-protein coupled receptor 105, GPRv53, Pfi-013, SP9144, HRH4, GPCR105

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