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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > Phospho-Thr202/Tyr204 ERK/MAPK Antibody

Phospho-Thr202/Tyr204 ERK/MAPK Antibody

Catalog # Availability Size / Price Inquiry
APR12847G 100 μl / $545

Phospho-Thr202/Tyr204 ERK/MAPK Antibody

Brand

Leading Biology

Catalog Number

APR12847G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Phospho-Thr202/Tyr204 ERK/MAPK antibody.

Molecular Weight

42/44 KDa

Host

Rabbit

Species Reactivity

Human, Rat

Target

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH.

GeneID

UniProt ID

Summary

Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).

Form

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC

Dilution

WB~~ 1:1000 IHC~~ 1:500

Synonyms

Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, Mapk1, Erk2, Mapk, Prkm1

Images

Western blot of human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202/Tyr204 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the phospho Thr202/Tyr204 ERK/MAP antibody. The immunolabeling is completely eliminated by treatment with λ-Ptase.

Immunostaining of granule cells in the dentate gyrus of saline treated mouse showing ERK/MAPK when phosphorylated at Thr202/Tyr204 (red) and nuclei (blue). Photo courtesy of Robert Wine.

Specification

Quantity

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