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Phospho-Ser778 Dynamin Antibody

Catalog # Availability Size / Price Inquiry
APR12836G 100 μl / $545

Phospho-Ser778 Dynamin Antibody

Brand

Leading Biology

Catalog Number

APR12836G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Phospho-Ser778 Dynamin antibody.

Molecular Weight

95 KDa

Host

Sheep

Species Reactivity

Rat

Target

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser778 conjugated to KLH.

GeneID

UniProt ID

Summary

Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.

Form

Prepared from sheep serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB

Dilution

WB~~ 1:1000

Synonyms

Dynamin-1, B-dynamin, D100, Dynamin, brain, Dnm1, Dnm

Images

Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser778 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser778 dynamin. The immunolabeling is completely eliminated by treatment with λ-Ptase.

Specification

Quantity

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