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GNL3 Antibody (N-term)

Catalog # Availability Size / Price Inquiry
APR17768G 100 μl / $495

GNL3 Antibody (N-term)

Brand

Leading Biology

Catalog Number

APR17768G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GNL3 antibody (N-term).

Molecular Weight

H=62, 61;M=61, 57;R=61 KDa

Cellular Localization

Antigen Cellular Localization: Nucleus. Nucleus, nucleolus. Note=Shuttles between the nucleus and nucleolus

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

38-72 aa

Target

This GNL3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 38-72 amino acids from the N-terminal region of human GNL3.

Symbol

E2IG3, NS

GeneID

UniProt ID

Function

May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).

Summary

May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

IF, WB

Dilution

WB~~1:1000 IF~~1:25

Synonyms

Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, Novel nucleolar protein 47, NNP47, Nucleolar GTP-binding protein 3, Nucleostemin, GNL3, E2IG3, NS

Images

All lanes : Anti-GNL3 Antibody (N-term) at 1:1000 dilution Lane 1: F9 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: K562 whole cell lysates Lane 4: Raji whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 62 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of Hela cells stained with GNL3 Antibody (N-term)( APR17768G). APR17768G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Specification

Quantity

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