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NCAM1 Antibody (C-term)

APR17822G Product Datasheet Protocol

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APR17822G		100 μl / $495

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Leading Biology Inc.

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NCAM1 Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR17822G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality NCAM1 antibody (C-term).
Molecular Weight	H=95,93;M=119,94;R=95 KDa
Cellular Localization	Antigen Cellular Localization: Isoform 1: Cell membrane; Single-pass type I membrane protein Isoform 3: Cell membrane; Lipid-anchor, GPI- anchor Isoform 5: Secreted.
Host	Rabbit
Species Reactivity	Human, Rat
Immunogen	796-830 aa
Target	This NCAM1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 796-830 amino acids from the C-terminal region of human NCAM1.
Symbol	NCAM
GeneID	4684
UniProt ID	P13591
Function	This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Summary	This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	FC, IHC-P, WB
Dilution	WB~~1:1000 IHC-P~~1:25 FC~~1:25
Synonyms	Neural cell adhesion molecule 1, N-CAM-1, NCAM-1, CD56, NCAM1, NCAM
Images	All lanes : Anti-NCAM1 Antibody (C-term) at 1:1000 dilution Lane 1: HT-1080 whole cell lysates Lane 2: SW480 whole cell lysates Lane 3: human cerebellum lysates Lane 4: human brain lysates Lane 5: rat brain lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 95 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Immunohistochemical analysis of paraffin-embedded H. astroglioma section using NCAM1 Antibody (C-term)( APR17822G). APR17822G was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. Flow cytometric analysis of SH-SY5Y cells using NCAM1 Antibody (C-term)(green, APR17822G) compared to an isotype control of rabbit IgG(blue). APR17822G was diluted at 1:25 dilution. An Alexa Fluor? 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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