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GLS Antibody (C-term)

APR07110G Product Datasheet Protocol

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APR07110G		100 μl / $495

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Leading Biology Inc.

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GLS Antibody (C-term)

Brand	Leading Biology	Catalog Number	APR07110G
Product Type	Polyclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GLS antibody (C-term).
Molecular Weight	73461 Da
Cellular Localization	Antigen Cellular Localization: Isoform 1: Cytoplasm, cytosol.
Host	Rabbit
Species Reactivity	Human, Mouse, Rat
Immunogen	516-545 aa
Target	This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.
Isotype	Rabbit Ig
Symbol	GLS1, KIAA0838
GeneID	2744
UniProt ID	O94925
Function	Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
Summary	Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.
Form	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB, IHC-P, IF, E
Dilution	WB~~1:1000 IHC-P~~1:25 IF~~1:25
Synonyms	Glutaminase kidney isoform, mitochondrial, GLS, K-glutaminase, L-glutamine amidohydrolase, GLS, GLS1, KIAA0838
Images	Western blot analysis of lysates from human brain tissue and Hela cell line (from left to right), using GLS Antibody (C-term)(Cat. APR07110G). APR07110G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane. Immunohistochemical analysis of paraffin-embedded H.kidney section using GLS Antibody (C-term)( APR07110G). APR07110G was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. Fluorescent image of Hela cells stained with XAF1 GLS Antibody (C-term)( APR07110G). APR07110G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).
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