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GPT Antibody (N-term R133)

Catalog # Availability Size / Price Inquiry
AMM05206G 100 μl / $495

GPT Antibody (N-term R133)

Brand

Leading Biology

Catalog Number

AMM05206G

Product Type

Polyclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GPT antibody (N-term R133).

Molecular Weight

54637 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

118-144 aa

Target

This GPT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-144 amino acids from the N-terminal region of human GPT.

Isotype

Rabbit Ig

Symbol

AAT1, GPT1

GeneID

UniProt ID

Function

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).

Summary

GPT(Glutamate-pyruvate transaminase), also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. This protein has 2 distinct molecular and genetic forms: one cytoplasmic (soluble) (GPT1) and one mitochondrial (GPT2). See ALTQTL1 and ALTQTL2 for information on quantitative trait loci influencing the plasma level of alanine aminotransferase.

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC-P, E

Dilution

IHC-P~~1:100 WB~~1:1000

Synonyms

Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, GPT 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT, AAT1, GPT1

Images

Immunohistochemical analysis of paraffin-embedded H.liver section using GPT Antibody (N-term R133)( AMM05206G). AMM05206G was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H.kidney section using GPT Antibody (N-term R133)( AMM05206G). AMM05206G was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

GPT Antibody (N-term R133) (Cat. AMM05206G) western blot analysis in U87-MG cell line , mouse liver ,and rat heart and liver tissue lysates (35ug/lane).This demonstrates the GPT antibody detected the GPT protein (arrow).

Specification

Quantity

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