> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Polyclonal Antibodies > IDH1 Antibody (Center)Brand |
Leading Biology | Catalog Number |
APR07923G |
Product Type |
Polyclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality IDH1 antibody (Center).
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Molecular Weight |
46659 Da
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Cellular Localization |
Antigen Cellular Localization:
Cytoplasm. Peroxisome
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Host |
Rabbit
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Species Reactivity |
Human, Mouse
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Immunogen |
116-143 aa
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Target |
This IDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-143 amino acids from the Central region of human IDH1.
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Isotype |
Rabbit Ig
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Symbol |
PICD
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GeneID |
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UniProt ID |
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Summary |
IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
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Form |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
IF, IHC-P, WB, FC, E
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Dilution |
IHC-P~~1:50~100
WB~~1:1000
IF~~1:10~50
FC~~1:10~50
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Synonyms |
Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH1, PICD
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Images |
Immunohistochemical analysis of paraffin-embedded H. prostate section using IDH1 Antibody (Center)( APR07923G). APR07923G was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from HepG2, MCF-7 cell line, human liver and rat liver tissue lysate(from left to right), using IDH1 Antibody (Center)( APR07923G). APR07923G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Confocal immunofluorescent analysis of IDH1 Antibody (Center)( APR07923G) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
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Specification |
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Quantity |
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| 6 | Leading Biology | APR03502G | IGHG1 Antibody (Center) | 100 μl | Polyclonal Antibodies |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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